Thank you for the response…
I have checked it and not found any zombie VMDK’s and there is no ISO Images on datastore. Even though, if any ISO Images are loaded and there should be not be spike in size from 10 to 50 GB because ISO will be maintain the standard size?
Let me check with the backup team and confirm whether any 3rd party storage or backup tools in place for this datastore. *** It’s a really good point****